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Image Search Results
Journal: Antibodies
Article Title: Development of a Novel Anti-CD44 Variant 8 Monoclonal Antibody C 44 Mab-94 against Gastric Carcinomas
doi: 10.3390/antib12030045
Figure Lengend Snippet: Anti-human CD44 mAbs production. ( A ) The structure of CD44s and CD44v. The mRNA of CD44s is assembled by the constant exons (1–5) and (16–20), and produces the standard isoform, CD44s. The mRNAs of CD44v are generated by the alternative splicing of variant exons. CD44v3–10 is an immunogen. CD44v3, 8–10, CD44v6–10, CD44v8–10, and CD44v3, 8 are detected in GC cell line . ( B ) PANC-1/CD44v3–10 was used as an immunogen. ( C ) The hybridomas were produced by fusion with splenocytes and P3U1 cells. ( D ) The screening was performed using parental CHO-K1 and CHO/CD44v3–10 cells by flow cytometry. ( E ) A clone C 44 Mab-94 was established. Furthermore, the binding epitope was determined by flow cytometry using CD44 deletion mutant-expressed CHO-K1 cells and ELISA.
Article Snippet: The
Techniques: Generated, Alternative Splicing, Variant Assay, Produced, Flow Cytometry, Binding Assay, Mutagenesis, Enzyme-linked Immunosorbent Assay
Journal: Antibodies
Article Title: Development of a Novel Anti-CD44 Variant 8 Monoclonal Antibody C 44 Mab-94 against Gastric Carcinomas
doi: 10.3390/antib12030045
Figure Lengend Snippet: Determination of C 44 Mab-94 epitope by ELISA. Four synthesized peptides, which cover the CD44v7 to v9 region, were immobilized on immunoplates. The plates were incubated with C 44 Mab-94 or buffer control (–1st Ab), followed by incubation with peroxidase-conjugated anti-mouse immunoglobulins. Optical density was measured at 655 nm. ELISA—enzyme-linked immunosorbent assay. NC—negative control (solvent; DMSO in PBS). Error bars represent means ± SDs.
Article Snippet: The
Techniques: Enzyme-linked Immunosorbent Assay, Synthesized, Incubation, Control, Negative Control, Solvent